DNase TREATMENT OF RNA To remove any contaminating genomic DNA from RNA isolates, we treated cell and CM samples with 1 U RNase-free DNase (Promega) per 1 [micro]g RNA in a 10-[micro]L reaction mixture including reaction buffer [supplied as 10x - 400 mmol/L Tris (pH 8.
What does RF stand for?
RF stands for RNase-Free
This definition appears frequently and is found in the following Acronym Finder categories:
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See other definitions of RF
We have 54 other meanings of RF in our Acronym Attic
- Right Front
- Right-Arm Fast (cricket; also seen as RAF)
- Righteous Fury (gaming, World of Warcraft)
- Riksidrottsförbundet (Swedish)
- Rim Fire (ammunition)
- Ripper Free (DVD backup software without ripping decoders)
- Ripping Friends
- Rising Force (Yngwie J. Malmsteen album)
- Risk Factor
- River Forest (suburb of Chicago, IL)
Samples in periodicals archive:
16 IU/[micro]l) of RNase-free certified DNase I with 10 IU/[micro]l.
Oncolumn DNase digestion was performed with 40 [micro]L of the enzymebuffer mixture from the RNase-Free DNase Set (Qiagen).
8 [micro]L QIAGEN OneStep RT-PCR Enzyme Mix, 4 [micro]mol/L of each primer and RNase-free water to 50 [micro]L.
4] in RNase-free water, and 10 [micro]L of diluted transcript was digested with 5 [micro]L of DNase I (3 U/[micro]L; RNase-Free DNase Set; Qiagen) for 30 min at 25[degrees]C in a total volume of 100 [micro]L that included 10 [micro]L of RDD buffer (RNase-Free DNase Set; Qiagen) and 5 [micro]L of RNase inhibitor (Applied Biosystems).
2], and RNase-free water in a final volume of 20 [micro]L.
In a final step, the beads were separated in the magnetic device, and the eluate was transferred to a fresh RNase-free tube.