Under alkaline conditions, fructosamine reverts to the eneaminol form, which reduces nitro blue tetrazolium to a formazan that can be quantified by measuring the absorbance (17).
What does BT stand for?
BT stands for Blue Tetrazolium (chemistry)
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We have 75 other meanings of BT in our Acronym Attic
- Blast Theory (band)
- Bleeding Time (test of blood)
- Block Termination
- Block Transfer
- Block Type
- Blood Test
- Blood Transfusion
- Blood Type
- Bloodthirster (gaming)
- Bloomington Public Transportation Corporation
Samples in periodicals archive:
Blots were stained for alkaline phosphatase using 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) (Jilani et al.
Superoxide radical scavenging assay The reaction mixture consisting of 1ml of nitro blue tetrazolium (NBT) solution (156 mM NBT in phosphate buffer, pH 7.
The superoxide anions were estimated by the spectrophotometric measurement of the product of the reduction of nitro blue tetrazolium (NBT).
Probes were visualized by bright field microscopy following formation of an oligoDIG-antiDIG-alkaline phosphatase conjugate treated with 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium.
The embryos were then blocked (1 hr) in 20% goat serum, incubated overnight in an antidigoxigenin antibody coupled to alkaline phosphatase (1:2,000) (Roche), washed extensively in PBS (at least 6 x 1 hr) and overnight in PBS, followed by a chromogenic nitro blue tetrazolium chloride (NBT) reaction [NBT/BCIP (5-bromo-4chloro-3-indolyl-phosphate, toluidine-salt) tablets, Roche] for 10-30 min, until color was visible.
5, with 4 [micro]L of nitro blue tetrazolium and 4 [micro]L of 5-bromo-4-chloro-3-indolyl phosphate) at room temperature until the developed bands were of the desired intensity, then the reaction was stopped by 200 [micro]L of 0.