The most widely used reagent is 2,4-dinitrophenylhydrazine (DNPH) which reacts with aldehydes to form hydrazones which are then determined by HPLC.
What does DNPH stand for?
DNPH stands for 2,4-Dinitrophenylhydrazine
This definition appears frequently and is found in the following Acronym Finder categories:
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We have 1 other meaning of DNPH in our Acronym Attic
- Department of Non-Public Education (Department of Administration; North Carolina)
- Detroit Name Plate Etching (est. 1911; Oak Park, MI)
- Di-n-pentyl ether (chemical compound)
- Division of Non-Public Education (North Carolina Department of Administration; also seen as NCDNPE)
- Dalat New Parents Forum
- Dan and Phil Games (UK)
- David Newham Property Group (South Africa)
- Digital Networks Product Group
- Dom Nine Pokorn Grmovje (Slovenian social welfare institution)
- Do Not Pass Go, Do Not Collect Two Hundred Dollars
- Décolletage Numérique des Plastiques Industriels (French: Digital Cutting of Industrial Plastics)
- Do Not Put in Mouth
- Dutch National Pathology Information System
- Dirección Nacional de la Policía Judicial (Spanish: National Directorate of Judicial Police; Ecuador)
- DNA-Dependent Protein Kinase
- Domain Name Parking (Internet)
- Drocourt Négoce Poids Lourds (French automobile retailer)
- Departamento Nacional da Produção Mineral (Brazilian: National Department of Mineral Production)
- Department of National Planning and Monitoring (Papua New Guinea)
- Bis (2,4-dinitrophenyl) oxalate (chemical)
Samples in periodicals archive:
Protein carbonylation assay To determine the carbonyl content of the tissue homogenates, the reaction between 2,4-dinitrophenylhydrazine (DNPH) and protein carbonyls was measured (Levine et al.
Sodium hydroxide, acetic acid, methanol, 2,4-dinitrophenylhydrazine (Brady's reagent, DNP), potassium iodide, ethyl acetate, hydrochloric acid, acetic anhydride, glacial acetic acid, perchloric acid, potassium hydrogen phthalate and Oracet blue indicator, Dragendorff's reagent and vanillin/sulphuric acid reagent were obtained from the Chemical Store of the Departments of Pharmaceutics and Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, KNUST, Ghana.
Following extraction, vitamin C concentration in the fermented products is quantified spectrophotometrically by the 2,4-dinitrophenylhydrazine method.
Plasma protein carbonyls--The content of protein bound carbonyls in plasma, an indicator of protein oxidation, was measured at 380 nm using 2,4-dinitrophenylhydrazine by the method of Levine et al (17).
This method can be used to assess the concentrations of oxidative stress via the increase in the formation of protein-bound carbonyls, which are measured based on the covalent reaction of the carbonylated protein side chains with 2,4-dinitrophenylhydrazine followed by detection with a specific antidinitrophenyl antibody (21).
Formaldehyde was analyzed as follows (18): A cartridge containing 2,4-dinitrophenylhydrazine (DNPH), which forms a derivative with formaldehyde, was connected to chamber outlets to measure the concentration of formaldehyde within the chamber.
Futhermore, volatiles of uncured pork and chicken, when passed through a 2,4-dinitrophenylhydrazine solution had a similar and a cured flavour note.