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cTnI quantification was based on the height of the cTnI peak and was interpolated from a calibration curve of peak height vs cTnI concentration derived from external calibrators prepared from purified human cTnI.
These features obviate the need to make tedious pre- and post-sample flow measurements or use an external calibrator to set low-flow rates.
Streamlined reagent formulation, multiplex assay format and inclusion of Armored RNA Quant[R] external calibrators improve the workflow and increase the number of specimens that can be tested per run.