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coli populations on the apples were enumerated at specified times by plating the bacteria on tryptic soy agar containing nalidixic acid.
The membranes are incubated at least three days on Tryptic Soy Agar (TSA), or on R2A (1) agar for at least five days, as specified by the European Pharmacopoeia (Ph.
We placed liver suspensions from the dead salamanders on Columbia agar with 5% sheep blood and tryptic soy agar and then incubated the samples up to 14 days at 20[degrees]C.
To prepare the broth culture of each organism, a loopful of cells was transferred aseptically from a tryptic soy agar (TSA) slant (Beckton, Dickinson and Co.
Working cultures of each of the above strains were maintained on Tryptic Soy Agar supplemented with 0.
Tryptic soy agar (TSA) and tryptic soy broth (TSB) were also purchased from Becton, Dickinson and Company.
Materials and Methods Serratia marcescens ATCC 14756 was grown on nutrient agar (NA), tryptone agar, and tryptic soy agar (TSA) (Difco) plates at 25[degrees]C and then a "lawn of growth" was inoculated to NA and TSA plates supplemented with one sugar (10% by weight/volume addition of glucose, sucrose, lactose, or sorbitol to traditional media) and incubated at 25[degrees]C, 30[degrees]C and 37[degrees]C for 24 hours.
A loopful from the overnight culture was grown for 24 h at 37[degrees]C on tryptic soy agar containing 50 mL/L sheep blood and on mannitol salt agar containing 6 mg/L oxacillin.