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The use of erythrocyte transketolase activity is an easy way of observing deficiency of thiamine pyrophosphate (TPP), and it has been found to be a frequently abnormal laboratory test that is guarantee of the biochemical lesion.
Transketolase is an enzyme that directs the precursors of advanced glycation end products (AGEs) to pentose phosphate pathway.
Thus, if the cell requires a significant amount of reducing power (NADPH), R5P will be converted back to F6P and GAP by transketolase generating NADPH.
Thiamine Deficiency and Flux Analysis of Transketolase Activity Thiamine, also known as vitamin B1, is essential for the optimal functioning and health of the cardiovascular, nervous, and digestive systems.
Measuring erythrocyte thiamine transketolase before and after the addition of thiamine pyrophosphate will detect a deficiency in thiamine.
A diagnosis of thiamine deficiency can be supported by decreased levels of erythrocyte transketolase, but it's important not to wait for lab results.
This study in Nature Medicine (published February 17, 2003 online edition) opens up the exciting possibility that easily absorbed lipid soluble thiamine effectively increases the activity of transketolase and thus may have additional therapeutic uses, including prevention and treatment of the side effects of diabetes.
Avemar makes this very difficult for cancer cells to do, because it inhibits the activity of the key enzyme in that pathway, transketolase (TK).