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Other screening methodologies which utilize PCR products such as RFLP and Thermal Gradient Gel Electrophoresis (TGGE) are prone to the same problem (Osborn & Moore 2000).
Denaturing gradient gel electrophoresis (7), single-strand conformation polymorphism analysis (8, 9), conformation-sensitive gel electrophoresis (10, 11), thermal gradient gel electrophoresis (12), enzymatic cleavage (13-15), and denaturing HPLC (DHPLC) (16) are examples of presequencing screening methods that have been developed to detect DNA variants.