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After PCR amplification, 5 [micro]L of each reaction was denatured at 95 [degrees]C for 10 min and added to hybridization solution [3 mol/L tetramethylammonium chloride, 50 mmol/L Tris-HCl (pH 8.
Tetramethylammonium chloride was tried because of its known property of eliminating the dependence of melting point ([T.
[FIGURE 1 OMITTED] Hybridization of the biotinylated PCR products to the five different ODN-coupled bead classifications was performed in tetramethylammonium chloride (TMAC) buffer (1X is 3 mol/L TMAC, 1 g/L sodium dodecyl sulfate, 50 mmol/L Tris-HCI, pH 8.