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The MALDI matrix was prepared by placing a saturated amount sinapinic acid in a 50% acetonitrile solution containing 0.
A thin layer of sinapinic acid solution (saturated solution in ethanol) was applied to a ground-steel MALDI target.
For intact protein analysis by MALDI-TOF-MS, sinapinic acid was used as the matrix.
Of particular interest is the presence of the sinapinic acid (SPA) adduct of the native TTR (peak c") and cysteinyl-TTR (peak e").
We applied a thin layer of sinapinic acid (saturated solution in ethanol) to a ground-steel MALDI target.
Equal volumes (1 [micro]L) of eluate and 50 mmol/L a-cyano-4-hydroxycinnamic acid or sinapinic acid matrix in 500 mL/L acetonitrile containing 1 g/L trifluoroacetic acid were mixed on a 384-position target plate and dried.
5 mg/L sinapinic acid (SPA) was added to each spot, dried, and then reapplied.
After air-drying, sinapinic acid matrix in 500 mL/L acetonitrile containing 5 mL/L trifluoroacetic acid was added to each array.