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Sinapinic acid was only found in the MeZA and PNRo, whereas caffeic, salicylic, and gallic acids were present in all wines.
The MALDI matrix was prepared by placing a saturated amount sinapinic acid in a 50% acetonitrile solution containing 0.
A thin layer of sinapinic acid solution (saturated solution in ethanol) was applied to a ground-steel MALDI target.
Sinapinic acid (3,5-dimethoxy-4-hydroxycinnamic acid) and [alpha]-cyano-4-hydroxycinnamic acid were the matrices used for proteins and peptides, respectively.
Of particular interest is the presence of the sinapinic acid (SPA) adduct of the native TTR (peak c") and cysteinyl-TTR (peak e").
We applied a thin layer of sinapinic acid (saturated solution in ethanol) to a ground-steel MALDI target.
Equal volumes (1 [micro]L) of eluate and 50 mmol/L a-cyano-4-hydroxycinnamic acid or sinapinic acid matrix in 500 mL/L acetonitrile containing 1 g/L trifluoroacetic acid were mixed on a 384-position target plate and dried.
5 mg/L sinapinic acid (SPA) was added to each spot, dried, and then reapplied.