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The most commonly used methods for quantification of gene expression include Northern blotting (1), ribonuclease protection assays (RPAs) (2), and reverse transcription-polymerase chain reaction (RT-PCR) (3, 4).
Total RNA were extracted from the treated cells and Northern blot or ribonuclease protection assays were performed, these experiments showed that the expression level of some of the ADAMs are modulated by growth factor treatment.
The ribonuclease protection assay (RPA) is commonly used to quantify levels of rare mRNAs in the cell.