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To isolate bacteria, we placed the swab samples into 5 mL of buffered peptone water (Difco APT broth; Becton Dickinson, Franklin Lakes, NJ, USA), incubated them for 24 h at 42[degrees]C with agitation, and then aliquots of the suspension were transferred into the following media: modified semisolid Rappaport-Vassiliadis basal medium (Oxoid, Sao Paulo, Brazil) with novobiocin (20 [micro]g/mL), selenite cysteine broth base (Oxoid), and xylose lysine desoxycholate agar (Difco XLD; Becton Dickinson).
5]) for subsequent inoculation of Rappaport-Vassiliadis (RV) broth (Eamens et al.
coli and Listeria) was formulated by scientists who compared its performance with other common selective media--modified EC broth with novobiocin for E coli O157:H7, Fraser broth for Listeria, and Rappaport-Vassiliadis broth for Salmonella.