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To avoid this limitation, I will use super-resolution fluorescence imaging methods such as direct Stochastic Optical Reconstruction Microscopy (dSTORM) and Photoactivated Localization Microscopy (PALM) combined with novel statistical cluster analysis to study quantitatively the dynamic reorganization of PM induced by protein trafficking, as well as the role played by actin-skeleton-induced compartments and lipid rafts during this process.
Photoactivated localization microscopy incorporates into a sample special fluorescent proteins that can be toggled between on and off states when hit with a particular wavelength of light.
Photoactivated Localization Microscopy and Stochastic Optical Reconstruction Microscopy