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Pellets were suspended in phosphate-buffered saline for further study.
1% Triton-X 100 in phosphate-buffered saline overnight.
Solutions of either ascorbic acid or the ascorbyl-2-phosphate analogs (at 10 mM) in phosphate-buffered saline (PBS) were relatively stable as shown by their decay rates and their ability to stimulate collagen synthesis even after nine days in solution prior to testing their effects on cultured cells.
Tissues were fixed in cold acetone for 5 minutes and in 1:1 acetone/ chloroform for 5 minutes and then washed with phosphate-buffered saline (PBS) three times for 3 minutes each time.
At necropsy, animals were perfused with Dulbecco's phosphate-buffered saline and the eyes were promptly removed.
Using the new method, the researchers could measure the size of the particles and their dispersion in solution, which in this case was a phosphate-buffered saline that is used as a simple model for blood.
They were ground in 20% fetal bovine serum-enriched phosphate-buffered saline in a Mixer Mill MM300 (Qiagen, Courtaboeuf, France) with one 3-mm tungsten bead and clarified by low-speed centrifugation.
After 30 min, cells were washed with phosphate-buffered saline (PBS) and fixed with Bouin's solution for 5 min.