What does PBSA stand for?

DNA purification was carried out by using Qiagen[R] buccal swab spin protocol with the following modifications: 1) a final concentration of 1 millimolar (mM) ethylenediaminetetraacetic acid (EDTA) was added to the phosphate-buffered saline extraction buffer; 2) the proteinase K treatment step was eliminated; and 3) an RNase mixture of A and T1 enzymes was used to degrade RNA after the final DNA purification step (Ambion Inc.

Solutions of either ascorbic acid or the ascorbyl-2-phosphate analogs (at 10 mM) in phosphate-buffered saline (PBS) were relatively stable as shown by their decay rates and their ability to stimulate collagen synthesis even after nine days in solution prior to testing their effects on cultured cells.

Fecal specimens from patients were diluted in phosphate-buffered saline (pH 7.

In addition, they exhibited bactericidal activity in phosphate-buffered saline.

Subsequently, the cells were cultured in serum-free medium for 24 h, and the medium was replaced by 2ml of phosphate-buffered saline.

Next, we needed a good solvent, so we used a mixture of phosphate-buffered saline that included 20 percent ethanol (alcohol) and 4 percent human albumin.

Two concentrations of histamine (1 mg/ml and 10 mg/ ml) were used as positive controls, and phosphate-buffered saline (PBS), the carrier substance in diluting the allergens, was used as the negative control.

Slides were then incubated for 25 minutes with linking solution (LSAB Kit, Universal, K0690, Dako Corporation, Carpinteria, Calif) and for 25 minutes with streptavidin-peroxidase, using phosphate-buffered saline washings between steps.