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The amino acids derivatives were determined by the Agilent HPLC system (Agilent 1100 Series, American), with autosampler and a Hypersil, octadecylsilane (ODs) column (C18, 250 x 4.
Briefly, the stationary phase used was an octadecylsilane column [C.
Briefly, we transferred an octadecylsilane (C18)coated paramagnetic bead suspension (5 [micro]L\ 50 g/L) to wells of 96-well plates containing 50 [micro]L serum, mixed the sample, and allowed it to stand for 1 min.
We injected 50 [micro]L of the supernatant into the chromatographic system [307 Pump HPLC (Gilson), FP 821 Fluorometer (Jasco), HP 3395 Integrator (Hewlett-Packard), and a Genesis octadecylsilane C18 column (150 x 4.
The separation of the ginsenosides was achieved with a reversed-phase Beckman ultrasphere C-18, 5 [micro]m octadecylsilane, 250 x 4.
75 V; Bioanalytical Systems), and a microbore column (Inertsil-2, 5-[micro]m octadecylsilane, 100 x 1.
We report here direct evidence of the absorption of anthocyanins in humans, which was obtained by combining an octadecylsilane (ODS) solid-phase extraction procedure for plasma sample preparation and an HPLC system with diode array for anthocyanin separation and detection.
column packed with 5-[micro]m particles of Shanden Hypersil octadecylsilane.