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In addition to the direct antioxidant properties, it has been found that NDGA is able to induce the nuclear factor erythroid 2-related factor 2 (Nr12) in primary cultures of cerebellar granule neurons (Guzman-Beltran et al.
Nuclear factor erythroid 2-related factors (NRFs), including NRF2 and NRF1, play critical roles in mediating the cellular adaptive response to oxidative stress.
The level of phosphorylated CREB, nuclear factor erythroid 2-related factor 2 (Nrf2), glutamylcysteine synthetase ([gamma]-GCS) and heme oxygenase 1 (H0-1) were measured by western blot method.