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The bacteria were enumerated on non-selective media.
faecalis were maintained for up to 48 h in brass and earthern vessels and enumerated on various selective and non-selective media either under (i) standard aerobic conditions, (ii) aerobic conditions in a growth medium supplemented with the peroxide scavenger sodium pyruvate, (iii) anaerobic conditions using unsupplemented medium; and (iv) anaerobic conditions in a growth medium supplemented with sodium pyruvate, the latter being regarded as ROS-neutralized conditions.
Conventional wisdom tells us to inoculate a specimen on the most enriched, non-selective media first and then proceed towards the most selective media.