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Concentrations were expressed as multiples of the median (MoM) for unaffected pregnancies of the same GA by means of 2 regression equations derived from the first- or second-trimester control groups.
Then, plasma concentrations of cf-mRNA for human PL and for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were measured and converted into multiples of the median (MoM) of the controls adjusted for gestational age, as described previously (5).
Results for each analyte were expressed in multiples of the median (MoM) for unaffected pregnancies of the same gestational age, derived from the regressed [log.
2 multiples of the median (MoM) but less than 3 MoM who then have a normal second sample do not have an elevated risk of fetal anomalies.