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The samples are ionized by bombarding the sample spray with electrons which are then separated according to mass-to-charge ratio.
The ionized analytes enter a flight tube and are separated by their individual mass-to-charge ratios, although because the analytes are typically singly charged with MALDI mass spectrometry, separation is effectively by mass alone.
Mass spectrometers identify the molecules present in a sample based on their mass-to-charge ratio (m/z).
Mass spectrometry measures the mass-to-charge ratio (m/z) of a molecule, which has been ionized, and its fragment ions.
Indeed, he says, initial SHS compounds with a mass-to-charge ratio (a kind of molecular fingerprint) of less than 370 were much reduced in the postreaction sample while many new compounds with mass-to-charge ratios of around 400 to 500 had formed.
Whether or not the biomarker is present in this more select sample can then be determined using mass spectrometry - a technique where the molecules are charged and identified from their mass-to-charge ratio.
The spectra showed a mass-to-charge ratio of 919 for the molecular ion, while the fragment peaks were shown at mass-to-charge ratios of 757, 465 and 303.
They include ion-chromatogram extraction for mass-to-charge ratio (m/z) analysis of the expected metabolites, isotope cluster analysis if the metabolites are expected to display unique isotopic patterns and comparative analysis against control samples.