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Influenza viruses were isolated in Madin-Darby canine kidney cells from lung tissue or nasal swab samples from pigs of various ages (young growers to adults) manifesting influenzalike illness (ILI) in Manitoba in January (A/ Swine/Manitoba/12707/05), Alberta in February (A/ Swine/Alberta/14722/05), British Columbia in May (A/Swine/British Columbia/28103/05), and Ontario in July (A/Swine/Ontario/33853/05).
A virus that was able to agglutinate rooster erythrocytes was isolated in Madin-Darby canine kidney cells from lung and bronchioalveolar lavage fluid of 1 of the 2 animals in which influenza virus was detected by IHC and RT-PCR.