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We used the Lowenstein-Jensen proportion method, recommended by the World Health Organization, according to the following critical drug concentrations: ofloxacin 3.
Individual bile acids were added to standard Lowenstein-Jensen (LJ) media to achieve physiological concentrations,4 as shown in Table 1.
AFb culture on Lowenstein-Jensen (LJ) medium grew Mycobacterium tuberculosis, while AFB staining was negative.
The preferred culture media involves lysis of peripheral blood leukocytes and inoculation onto solid media (eg, Lowenstein-Jensen, Middlebrook 7H11 agar) or into radiometric broth.
Lowenstein-Jensen medium, or in liquid culture using the BD MGIT[TM] system.
Nucleic acid amplification assays and cultures performed on mycobacteria growth indicator tube (Bactec MGIT; Becton Dickinson, Franklin Lakes, NJ, USA) and on Lowenstein-Jensen medium were positive for Mycobacterium tuberculosis isolates that later showed sensitivity to streptomycin, isoniazid, rifampin, and ethambutol.
We compared the performance of the COBAS TaqMan MTB assay (Roche Molecular Systems, NJ, USA) against conventional Lowenstein-Jensen (L-J) culture and Mycobacterial growth indicator tube (MGIT) 960 (Beckton Dickinson, Maryland, USA) an automated culture system for diagnosis of Mycobacterium tuberculosis.
Culture methods Lowenstein-Jensen medium has usually been used for culture of M.