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The Simple Plex platform will join other innovative instrument platforms which include instruments for automated Western blot analysis, devices for isoelectric focusing of proteins and protein particle analysis.
The scientists used isoelectric focusing to estimate the inhibitor's isoelectric point: 7.
The authors studied cord blood serum proteins by profiling a UCB pool of 12 neonates with different backgrounds using a combination of isoelectric focusing and liquid chromatography coupled with matrix-assisted laser desorption/ionization tandem mass spectrometry and by comparing results with metabolic and disease databases available for adult blood.
Isoelectric focusing was performed on freeze-thaw extracts on polyacrylamide gels as described (4).
This hemoglobin variant was detected by isoelectric focusing moving just ahead of Hb A.
Coverage includes common microfabrication techniques utilized to create microfluidic devices and on-chip flow control and mixing microsystems, on-chip electrophoresis and isoelectric focusing methods for quantitative biology, electrical methods for manipulations of droplets via electrowetting and particles via dielectrophoresis for separations and chemical reactions, methods for integrated optical characterization of microfluidic devices and for controlling chemical gradients within devices, microimmunoassay diagnostics, MicroFACS system, and microtubule motors in microfluidics.
3) A Task Force report from the European Federation of Neurological Science (EFNS) recently described Class I evidence to support the use of isoelectric focusing for both predictive and diagnostic testing in the diagnosis of MS.
Capillary isoelectric focusing (clEF) is another accurate method where the sensitivity of detection of capillary electrophoresis is combined with the automated sampling and data acquisition of HPLC (5,6).