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5 mM isobutylmethylxanthine (IBMX), 100 [micro]M indomethacin, 10% FBS and insulin (10 [micro]g/m1), for 7 days.
The media was then changed to DMEM supplemented with 10% FBS as described above and the following hormones were added: insulin (10 g/ml), dexamethasone (1 M), indomethacin (200 M) and isobutylmethylxanthine (0.
Platelets are stabilized by increasing intracellular CAMP with forskolin and by inhibiting CAMP phosphodiesterase with isobutylmethylxanthine (IBMX) in the stabilizing solution.