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Serum electrophoresis showed no M band, but serum immunofixation electrophoresis was positive for amyloid light chain.
Immunofixation electrophoresis (IFE) revealed a monoclonal IgA [kappa] protein.
A urine and serum immunofixation electrophoresis revealed a monoclonal gammopathy of free [kappa] light chain.
68 g/L IgM) had returned to normal levels, and immunofixation electrophoresis revealed that the monoclonal heavy chain had disappeared.
Serum immunofixation electrophoresis revealed the presence of a monoclonal [gamma] band with no corresponding light chain (Fig.
The patient was subsequently referred to our institution for treatment options, where additional serum protein electrophoresis (Beckman Coulter PARAGON system, Brea, Calif) and serum immunofixation electrophoresis (Beckman Coulter) analyses confirmed the presence of an IgG[Kappa] M-protein that had increased to 60 g/L.
Interestingly, we estimated a concentration of 100-200 mg/L for this paraprotein with SPE and immunofixation electrophoresis, a result consistent with the conclusion of de Kat Angelino et al.
Siltuximab was evident as an IgG[kappa] monoclonal protein at a threshold of approximately 100 mg/L and was detected by all methods tested, including capillary electrophoresis, immunosubtraction (not shown), agarose gel electrophoresis, and immunofixation electrophoresis (Fig.