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Specific topics include the isolation and primary culture of human proximal tubule cells, the laser capture micro-dissection of kidney tissue, immuno and lectin histochemistry for renal light microscopy, identifying apoptosis in kidney tissue sections, and mechanical stretch-induced signal transduction in cultured mesangial cells.
9:00 CADMIUM INDUCED ALTERATION OF CADHERIN EXPRESSION IN THE MT-3 TRANSFECTED HUMAN PROXIMAL TUBULE CELL LINE HK-2, Chandra S Bathula; Scott H.
It was shown that, an immortalized human proximal tubule cell line HK-2 does not form domes and further studies had indicated that this cell line also does not express the Metallothionein-3 isoform that is normally expressed in the proximal tubules of the kidney in-situ as well as in cell cultures.