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48% in HEPES buffer at a physiological chloride concentration of 100 mmol/L, 0.
The liver was isolated under aseptic conditions and placed in chilled HEPES (N-2-hydroxyethylpiperazine-N-2 ethane sulphonic acid) buffer containing HEPES (0.
The follicular contents were pooled into 50 ml tubes and allowed to sediment, the sediment was placed into HEPES buffered Tyrode-lactate medium (TL-HEPES-PVA; 114 mM NaCl, 3.
The new MHM[TM] product is the first dual buffered solution that provides a safe and secure environment for gametes and embryos under ambient conditions because of its unique buffering capacity using both HEPES and MOPS buffers, in addition to select amino acids.
4] DNA ligase (New England Biolabs) were deadenylated for 30 min at room temperature in 50 mmol/L HEPES, pH 7.
The TriFECTa kit includes: --Three target-specific Dicer-Substrate siRNA duplexes (5 nmoles each) --Cy3TM DS Transfection Control, fluorescent-labeled transfection control duplex (1 nmole) --HPRT-S1 DS Positive Control duplex (1 nmole) --DS Scrambled-Neg, universal negative control duplex (1 nmole) --RNase Free Duplex Buffer (100mM KAc/30 mM HEPES pH 7.
1) at 37[degrees]C by the HEPES contained in the dialysis buffer (6).
Dulbecco's modified Eagle's medium (DMEM), RPMI-1640, fetal calf serum (FCS), HEPES, L-glutamine, 2-mercaptoethanol, penicillin/streptomycin, bovine serum albumin (BSA), dinitrophenyl conjugated human serum albumin (DNP-HSA), p-nitrophenyl-N-acetyl-[beta]-D-glucopyranoside, and neutral red were obtained from Sigma Chemical Company (St.