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Nodules were removed, counted and sliced cross-sectionally for assessment for potential BNF using the leghaemoglobin pigment indicator Thereafter, each sample's nodules gravimetric moisture content and eventual dry nodule dry weight was determined.
The bulk density and the gravimetric moisture content were determined for the 20 samples and the volumetric moisture content was determined by multiplying the gravimetric moisture content by the bulk density.
The main goal of the study reported here was to develop procedures to produce good estimates of in situ gravimetric moisture content.
In addition, 3 small samples (25 g of soil) were taken from the upper 10 mm of the soil in each of the 2 pots in Box 1 for gravimetric moisture content determination.