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2]) inhibits synthesis of proteoglycan structure in cartilage via adenosine triphosphate synthesis and inhibition of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme in chondrocytes.
After blocking with 5% skim milk in TBST, the membranes were incubated with primary antibodies against the following: Nrf2, NQ01, and histone 1 (Santa Cruz); I3-actin (Sigma); OxPhos Complex I subunit NDUFS3, OxPhos Complex II 30 kDa subunit, OxPhos Complex ll 70 kDa subunit, OxPhos Complex III subunit core 1, OxPhos Complex IV subunit I, and OxPhos Complex V (Fl FO-ATP synthase) subunit a (Invitrogen); and pyruvate kinase M2, lactate dehydrogenase (LDHA) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cell Signaling) at 4 [degrees]C overnight.
METHODS: In male Sprague-Dawley rats (n = 8/group; 10d juice intervention, 4d wash-out; 4 treatment cycles), expression of target genes (superoxide dismutase, SOD1/SOD2; glutathione peroxidase, GPX1/GPX2; [gamma]-glutamylcysteine ligase, GCLC/GCLM; glutathione reductase, GSR; catalase, CAT; NAD(P) H:quinone oxidoreductase-1, NQO1 and transcription factor erythroid-derived 2-like-2, Nrf2) was quantified with duplex RT-PCR, using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as control.
We then used semiquantitative RT-PCR with glyceraldehyde-3-phosphate dehydrogenase as an internal reference to detect the virus and determine the amount of the viral RNA in the medium.
The glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, was down-regulated (Lau et al.
49 G6PDH-1 * G6PDH-2 * Glyceraldehyde-3-phosphate dehydrogenase 1.
F-actin has been shown to bind glycolytic enzymes like fructose-l,6- biphosphate aldolase (aldolase) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
brucei, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), was also evaluated for betulinic acid, olenaolic acid, ursolic acid.