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2004) as redox potential sensors with two fluorescence excitation maxima, thus permitting ratiometric analysis that minimizes errors associated with variations in indicator concentration (roGFP expression), illumination intensity, and cell thickness.
The absorbance, fluorescence excitation, and MISF spectra of a fecal specimen from a pregnant woman analyzed by these three methods are shown in Fig.
Both systems offer the choice of fluorescence excitation and detection wavelengths.
The system would utilize the TE-2000's one-of-a-kind second filter cube assembly that can separate the optical paths of the laser microsurgery beam and the fluorescence excitation light.
The time-resolved fluorescence excitation and emission spectra of nanoparticles (3 pmol/L) in 1 mL/L Triton X-100 and the spectra of europium ions (1 nmol/L) in the enhancement solution were measured with the LS5 spectrofluorometer (Perkin-Elmer Instruments).
Figure 1 shows the fluorescence excitation and emission spectra of DASB in THF.
This action instigates microfluidic flow control, thermocycling, cell lysis, mixing and fluorescence excitation, all of which are basic functions involved in performing complex biochemical analysis.
The dyes match every commonly used fluorescence excitation source and set of emission filters, from the ultraviolet to the near infrared, permitting end users to develop optimized multiplex assays using direct fluorescence detection, fluorescence polarization assays, or fluorescence resonance energy transfer (FRET) methods.