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Mohammad Reza Hormozinejad (assistant professor of Department of Chemistry) and his colleagues proposed a simple, highly sensitive, and selective method for the quantitative measurement of captopril in human blood plasma by using fluorescein isothiocyanate (FITC) modified with gold nanoparticles.
specific antibody CRY104 labeled with fluorescein isothiocyanate (Biotech Frontiers, North Ryde, Australia) for enumeration.
For FISH analyses, hybridization using vertebrate telomere peptide nucleic acid (PNA) probes (Telomere PNA FISH Kit/ fluorescein isothiocyanate [FITC]; DakoCytomation, Glostrup, Denmark) was performed according to the manufacturer's instructions with some modifications and as described in detail by Sakai et al.
Cells were fixed and permeabilized using a commercial kit (Cytofix/ Cytoperm; PharMingen, San Diego, CA, USA) and stained for intracellular TNF-[alpha] using fluorescein isothiocyanate (FITC)-conjugated rat anti-mouse TNF-[alpha] Ab (PharMingen).
The fluorometer is used to measure the fluorescence signal of a suspension of microspheres with immobilized fluorescein isothiocyanate (FITC).
The D3 Duet(TM) DFA kits use brighter, easily distinguishable fluorescent stain colors to improve interpretation of virus-infected cell cultures by combining monoclonal antibodies labeled with either Fluorescein Isothiocyanate (FITC) or R-Phycoerythrin (R-PE) in one reagent for simultaneous detection.
After washing, these cells were first incubated with a monoclonal anti-insulin antibody (1:200 dilution; Sigma, Madrid, Spain) for 2 hr and then for 1 hr with a secondary anti-body, anti-mouse IgG-conjugated fluorescein isothiocyanate (IgG-FITC; 1:200 dilution; Sigma), both at room temperature.
Biotin-labeled DNA was detected by use of avidin-conjugated fluorescein isothiocyanate (FITC; Vector) and biotin-conjugated avidin (Vector).