The blood samples were centrifuged at 1000 g 4 [degrees]C for 5 min, then the supernatant was collected and stored at 70 [degrees]C for measurement of cytokines, and sediment was used to separate PBMCs by standard Ficoll-Hypaque (Pharmacia, Uppsala, Sweden) density centrifugation.
What does FH stand for?
FH stands for Ficoll-Hypaque
This definition appears very rarely and is found in the following Acronym Finder categories:
- Science, medicine, engineering, etc.
See other definitions of FH
We have 33 other meanings of FH in our Acronym Attic
- Faith Hill (musician)
- Familial Hypercholesterolemia (genetic disorder)
- Family History
- Family Housing
- FarmHouse (fraternity)
- Fédération de l'Horlogerie (French: Watchmaking Federation)
- Fellowship Hall
- Fermanagh (Ireland)
- Fetal Heart
Samples in periodicals archive:
PBMC were isolated using Ficoll-Hypaque PLUS (GE healthcare) following the manufacturer's instructions.
PBMCs were obtained from HIV-1-infected individuals and HCs by Ficoll-Hypaque density gradient centrifugation, cryopreserved in fetal calf serum supplemented with 10% DMSO, and stored in liquid nitrogen within 8hof collection.
Blood was collected into two heparin-containing Vacutainer tubes, and all blood samples were temporarily kept at 4 [degrees] C until transported to the local laboratory for isolation of PBMCs using a standardized Ficoll-Hypaque gradient procedure.
Heparinized whole blood was obtained from healthy volunteers and peripheral blood mononuclear cells (PBMCs) immediately separated using conventional Ficoll-Hypaque (GE Healthcare, Uppsala, Sweden) density gradient centrifugation.
PMN purification, culture and injection: Human PMN were isolated from the heparinized venous blood by Ficoll-Hypaque (Amersham Biosciences, USA) gradient centrifugation followed by sedimentation in 3 per cent Dextran (1) (Sigma Chemicals, USA).
Peripheral blood mononuclear cells (PBMCs) were derived from peripheral blood by Ficoll-Hypaque separation and evaluated for NKCA (Beno, Stover, & Mathews, 1995).
The mononuclear cells from the whole blood were obtained using the Ficoll-Hypaque separation procedure (8, 9).