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We then added 150 [micro]L (105 cells/mL) of a Vero cell suspension with 5% fetal calf serum.
The medium was replaced with DMEM containing 5% fetal calf serum and 5 [micro]g/ml of insulin, with or without 5 [micro]g/ml of dexamethasone after reaching subconfluence.
A549 and MRC-5 cells were incubated in EMEM with 2% fetal calf serum.
5]) (Kiderlen and Kaye, 1990) in 200 [micro]l RPMI 1640 medium supplemented with 5% heat-inactivated fetal calf serum (herein designated R5) were incubated for 48 h with test compounds (50 [micro]g/ml) or medium alone before further fetal calf serum (10% final concentration) was added.
26 Kyodo A team of Japanese scientists has successfully extracted a previously undiscovered substance from fetal calf serum which can protect neurons of the central nervous system (CNS) from harmful endogenous materials.
On the other side was a special mixture of fetal calf serum that acted as an attractant to the neutrophils.
In 2004, an atypical pestivirus, D32/00_HoBi, distantly related to BVDV-1 and BVDV-2 was isolated from a batch of fetal calf serum collected in Brazil (3).
Cells were grown in phenol red-free EMEM containing 2 mM glutamine, 1% nonessential amino acids, 100 U/mL antibiotics (mix of penicillin, streptomycin, and fungizone), 1 mM sodium pyruvate, and 10% fetal calf serum (Biowhittaker, Gagny, France).