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HCAECs were cultured in endothelial cell medium (ECM) supplemented with endothelial cell growth supplement (ECGS) (Carlsbad, CA, USA) at 37 C in a 5% CO2 and 95% ambient air environment.
Endothelial cells were isolated by using BS-1 lectin-coated magnetic beads, and cultured in a selective medium containing DMEM, endothelial cell growth supplement, D-valine, heparin, and plasma-derived horse serum.