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Many different prescreening methodologies have been applied to breast cancer genes, including denaturing gradient gel electrophoresis (6, 7), single-strand conformation polymorphism analysis (8, 9), hetero-duplex analysis (10), conformation-sensitive gel electrophoresis (11,12), denaturing HPLC (13), and mismatch-cleavage assays (14).
Detection of the -119G>A substitution by conformation-sensitive gel electrophoresis has recently been proposed (5).
HA by conformation-sensitive gel electrophoresis (CSGE) (8) is considered to detect almost 100% of sequence variants and is suitable for high-throughput designs.