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An evaluation of the overestimation of "acute phase reactants" by use of the bromcresol green reaction.
The purpose of this study was to examine the validity of albumin determinations in penguin plasma by the bromcresol green (BCG) method and the gold standard of protein electrophoresis (EPH).
BAL fluid albumin was measured colorimetrically at 628 nm based on albumin binding to bromcresol green using a commercial assay kit (Albumin BCG diagnostic kit; Sigma Chemical Co.
Albumin was measured for 142 specimens using a Hitachi 917 analyzer with bromcresol green reagent from Roche.
Albumin was measured by a direct colorimetric procedure using the specific dye bromcresol green (19).
Albumin is most commonly measured by dye-binding methods, particularly bromcresol green and bromcresol purple; currently, ~50% of laboratories use each method.
When we checked the accuracy of the Vitros "dry chemistry" bromcresol green (BCG) albumin method (Ortho-Clinical Diagnostics), the albumin assay used at our hospital, by analyzing our routine blood bank albumin solution (Octapharma), we found only 70% of the expected value.
Under these conditions, results of the bromcresol green (BCG) methods can be falsely inflated (9-15) and bromcresol purple-binding methods prone to falsely lowered results (16), although reagent manufacturers have adopted many variations of the dye-binding albumin methodology in an attempt to assure accuracy.