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The purpose of this study was to examine the validity of albumin determinations in penguin plasma by the bromcresol green (BCG) method and the gold standard of protein electrophoresis (EPH).
Discordant results in the measurement of serum albumin concentrations have long been recognized with various assays, including agarose gel protein electrophoresis (PEL),  bromcresol green (BCG), and bromcresol purple (BCP) (1,2).
BAL fluid albumin was measured colorimetrically at 628 nm based on albumin binding to bromcresol green using a commercial assay kit (Albumin BCG diagnostic kit; Sigma Chemical Co.
Albumin is most commonly measured by dye-binding methods, particularly bromcresol green and bromcresol purple; currently, ~50% of laboratories use each method.
When we checked the accuracy of the Vitros "dry chemistry" bromcresol green (BCG) albumin method (Ortho-Clinical Diagnostics), the albumin assay used at our hospital, by analyzing our routine blood bank albumin solution (Octapharma), we found only 70% of the expected value.
Under these conditions, results of the bromcresol green (BCG) methods can be falsely inflated (9-15) and bromcresol purple-binding methods prone to falsely lowered results (16), although reagent manufacturers have adopted many variations of the dye-binding albumin methodology in an attempt to assure accuracy.
Plasma albumin (measured by bromcresol green on the Paramax RX analyzer) also decreased linearly with increasing concentrations of NaF, confirming that the change in hematocrit was not artifactual (Table 1).
The Hitachi measures albumin by monitoring the interaction between albumin and bromcresol green (BCG), a dye specific for albumin.