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Amplification Methods - PCR - Ligase Chain Reaction - Branched DNA - Q-Beta Replicase - NASBATable of Contents (continued) - SDA - 3 SR - HPA - Two-Tiered System - LAT Artificial Intelligence Dry Chemistry Biosensors Competitive Profiles - Abbott - ADI/American Diagnostica - Axis-Shield - Beckman Coulter/Danaher - Becton Dickinson - Bio/Data Corporation - Biosite/Inverness - Chrono-Log - Corgenix Medical - Diagnostica Stago/Trinity Biotech - Helena Laboratories - Hyphen BioMed - Instrumentation Laboratory - ITC/Nexus Dx - Roche - Siemens - Sienco - Sysmex - Thermo Fisher - ZyCare/Alere To order this report: In_Vitro_Diagnostic Industry : Analysis of Hemostasis Testing Technologies And Strategic Profiles of Leading Suppliers Contact Nicolas: nicolasbombourg@reportlinker.
This assay is based on branched DNA (bDNA) signal amplification technology and enables quantification of p53-like mRNA levels in as few as 2,500 hemocytes, with a coefficient of variation close to 6% (range, 2-12%).
Some of these include branched DNA, hybrid capture, transcription-mediated amplification, cleavase-based assays, home-brew fluorescence in situ hybridization assays (where the DNA of interest is ordered using public genomic databases and fluorescently labeled in house), Invader assay, strand displacement assays and pyrosequencing.
These tests, using polymerase chain reaction (PCR) or branched DNA (bDNA) technology, offered a more direct method of measuring the treatment effect on the virus in blood, as well as other tissue compartments, compared to measuring p24 antigen or CD4 cells.
Human immunodeficiency virus RNA was detected using a branched DNA amplification technique as described by Collins et al, (3) except detection was automated on the Ventana Gen II (Ventana Medical Systems).
The guidelines advise considering treatment in these patients if the plasma viral load of HIV RNA rises to 30,000 copies/mL, as measured by the branched DNA test.
These modified branching non-nucleoside bases, which BioGenex refers to as "diverge DNA", avoids the problem of non-specific hybridization that is seen with branched DNA signal amplification and detection.
Amplification Methods Polymerase Chain Reaction Robotics Temperature Cyclers PCR Variations Immuno-PCR QC-PCR DAP-PCR Ligase Chain Reaction Branched DNA Q-Beta Replicase Nucleic-Acid Sequence-Based Strand Displacement Activation Self-Sustained Sequence Replicase 2.