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Stock viruses were prepared by infection of baby hamster kidney cells for PRY, infection of Madin Darby bovine kidney cells for BVD, and infection of chronically infected mink lung cells for XMuLV For each virus, the cytotoxicity and potential interference with the infectivity assay were determined.
IMMUNOBLOT ANALYSIS OF RENAL AND URINARY TREHALASE A specimen of a human kidney cortex-mix obtained from a nephrectomized patient with renal carcinoma and sections of porcine and bovine kidneys were used for immunoblotting.
For the virologic examination, samples were added to confluent monolayers of Madin-Darby bovine kidney cells, observed daily for cytopathic effect, and passaged every 7 days.