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The polymerase enzyme sub-segment is divided into high fidelity DNA polymerase, T4 DNA polymerase, Taq DNA polymerase, and other polymerases, while the restriction endonuclease enzyme sub-segment is divided into BamHI, EcoRI, PstI, and other restriction endonucleases.
The restriction endonucleases enzyme segments are sub-segmented into BamHI, EcoRI, PstI, and other restriction endonucleases.
DNA in the plugs was digested with 40 U of XhoI (New England Biolabs, Ipswich, MA, USA) or 40 U of BamHI (New England Biolabs), for 16 and 3 h, respectively, at 37[degrees]C following the manufacturer's protocol.
Then 20ul HindIII and BamHI reaction was controlled by electrophoresis in a 0.
1 g/L), 100 U each of RsaI, EcoRI, BamHI, and EcoRV (all 4 restriction enzymes from New England Biolabs).
AT 49, Anup Bamhi is one of the few shopcum- residence owners at Khan Market who has not succumbed to commercial pressures.
Wu, John Hopkins Medical Institutions, USA) using BamHI and HindIII and the resulting DNA fragment was gel purified according to manufacturer details (QIAquick gel extraction kit protocol, QIAGEN, USA).
The Tat5910 sense (5'-gat ccA AGC AAC ACT TTT CTG ATG AGT CCG TGA GGA CGA AAC AAT AGC AAT TGG TAC Aa- 3') and antisense sequences (5'-agc ttT GTA CCA ATT GCT ATT GTT TCG TCC TCA CGG ACT CAT CAG AAA AGT GTT GCT Tg- 3') were synthesized with BamHI and HindIII sites to facilitate cloning.