Antimicrobial Susceptibility Testing Susceptibility to antimicrobial agents was tested by means of the disk diffusion method according to the National Committee for Clinical Laboratory Standards (NCCLS) guidelines (NCCLS, 2000) for the following antimicrobials: amoxicillin/clavulanic acid (AMC, 30 [micro]g), ampicillin (AMP, 10 [micro]g), apramycin (APR, 15 [micro]g), cephalothin (CEF, 30 [micro]g), chloramphenicol (CHL, 30 [micro]g), enrofloxacin (ENR, 5 [micro]g), gentamicin (GEN, 10 [micro]g), kanamycin (KAN, 20 [micro]g), nitrofurantoin (NIT, 300 [micro]g), streptomycin (STR, 10 [micro]g), tetracycline (TET, 30 [micro]g), and trimethoprim/sulfamethoxazole (SXT, 25 [micro]g), all supplied by Oxoid.
Sensititer (TREK Diagnostic Systems, LTD, West Sussex, England), a commercially prepared dehydrated panel, was used for the following antimicrobial agents: amoxicillin-clavulanic acid, ampicillin, apramycin, ceftiofur, chloramphenicol, ciprofloxacin, colistin, florphenicol, gentamicin, nalidixic acid, neomycin, streptomycin, sulfamethoxazole, tetracycline, and trimethoprim.
Newport isolates were tested for resistance to amikacin, ampicillin, amoxicillin/clavulanic acid, apramycin, cefoxitin, ceftiofur, ceftriaxone, cephalothin, chloramphenicol, ciprofloxacin, gentamicin, kanamycin, nalidixic acid, streptomycin, sulfamethoxazole, tetracycline, and trimethoprim/sulphamethoxazole by using the Trek Diagnostics Sensititre CMV1UIL (Trek Diagnostic Systems, Inc.
Sensitivity testing of chosen isolates (MICs) was conducted by using a panel of 17 antimicrobial agents (amikacin, amoxicillin-clavulanic acid, ampicillin, apramycin, ceftiofur, ceftriaxone, cephalothin, chloramphenicol, ciprofloxacin, gentamicin, kanamycin, nalidixic acid, streptomycin, sulfamethoxazole, tetracyline, ticarcillin, and trimethoprim-sulfamethoxazole) on the Sensititre system (TREK Diagnostic Systems, Westlake, OH).
For example, because it has an unusual structure, apramycin (a 4-substituted-2-deoxystreptamin) was used exclusively in animals in the hope that it would not be recognized by any of the known aminoglycoside-modifying enzymes (3).