Many methods have been used to detect C282Y, including oligonucleotide ligation assays (1), fragment length polymorphism analyses of PCR products after restriction enzyme digestion (5), allele-specific oligonucleotide hybridization assays (2), mutagenically separated PCR assays (10), primer extension assays (11, 12), and allele refractory mutation systems (13, 14).
What does ASOH stand for?
ASOH stands for Allele-Specific Oligonucleotide Hybridization (clinical chemistry)
This definition appears somewhat frequently and is found in the following Acronym Finder categories:
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- Access Service Ordering Guidelines
- Access Service Ordering Guidelines (telecommunications)
- Air Support Operations Group
- American Security Officers Guild
- Analytical Solution of Groups (chemistry)
- Ateneo School of Government (Graduate School of Leadership and Public Service; Ateneo de Manila University; Quezon City, Philippines)
- Avenger/Sebring Owners Group
- A Show of Hands (Rush album)
- A Sort of Homecoming (U2 song)
- Acute Surgical Orbital Haemorrhage (eye surgery complication)
- Amazing Screw-On Head (TV show)
- Australian School of Herbal Medicine (Ridgehaven, South Australia)
- Australian Society of Otolaryngology Head and Neck Surgery
- Abdominal Solid Organ Injury (blunt trauma)
- After Start of Injection (combustion dynamics)
- American Society of Inventors (Philadelphia, PA)
- Association Suisse d'Osteogénèse Imparfaite
- Aveyron Serre Olt Initiative (French business lending initiative)
- A Song of Ice and Fire (Fantasy Series by George R.R. Martin)
- Associated Items of Equipment
Samples in periodicals archive:
Multiplex PCR with allele-specific oligonucleotide hybridization was another combined approach (30).
In this field, the combination of solid supports bearing a set of immobilized oligodeoxynucleotide (ODN) probes with the allele-specific oligonucleotide hybridization reaction is a useful tool.
PCR with allele-specific oligonucleotide hybridization (11, 14, 25), amplification with sequence-specific primers (26-29), ASRA (18, 30), and single-strand conformation polymorphism (31) have been used to genotype the PAI1 promoter polymorphism.
Many methods have been reported for the study of BRCA mutations, including allele-specific oligonucleotide hybridization (8, 9), allele-specific PCR (10), PCR-mediated site-directed mutagenesis (11,12), heteroduplex analysis (HDA) (13-15), single-strand conformation polymorphism (14,16), and the protein truncation test (14,15).
PiZ and PiS genotypes may be determined by DNA-based methods such as restriction fragment length polymorphism, allele-specific oligonucleotide hybridization, allele-specific amplification, direct sequencing, dual-color detection by ligase-mediated analysis, temperature or denaturing gradient gel electrophoresis, and PCR-mediated site-directed mutagenesis (11-19).
They are investigated by various methods, including restriction enzyme digestion, direct sequencing, use of an amplification refractory mutation system, and allele-specific oligonucleotide hybridization (4).
The oligonucleotide probes used for allele-specific oligonucleotide hybridization were essentially those described by Owerbach et al.