What does DNPH stand for?

2'-7'-dichlorofluorescein diacetate (DCFH-DA), 2'-7'-dichlorofluorescein (DCF), trichloroacetic acid (TCA), 2,4-dinitrophenylhydrazine (DNPH), guanidine hydrochloride, phenylmethylsulfonyl fluoride (PMSF), 5-amino-2,3-dihydro-l,4-phtalazinedione (luminol) and hydrogen peroxide stock solution were purchased from Sigma Chemical Co.

With respect to the used base concentration, the developed spectrophotometric procedure with p-DMAB benzaldehyde derivative was found to be more practically appropriate than the modified Randle reference method, which is a very common used spectrophotometric method for the analysis of pyruvic acid in onions via the utilization of 2,4-dinitrophenylhydrazine (DNPH) color agent [35].

Briefly, 10 [micro]L mitochondrial protein was derivatized with 2,4-dinitrophenylhydrazine and blotted to polyvinylidene fluoride membrane (Amersham Biosciences, Piscataway, NJ).

The determination of ascorbic acid in bile, blood and urine through 2,4-dinitrophenylhydrazine derivatives of dehydro-ascorbic acid.

Protein oxidation was measured in brain, gill, and liver by detecting carbonyl moieties using 2,4-dinitrophenylhydrazine (DNPH) and normalized to total protein.

Briefly, after ozone exposure, samples were derivatized with 2,4-dinitrophenylhydrazine (DNPH).

3 L/min with 2,4-dinitrophenylhydrazine as an adsorbent.

Briefly, we used cartridges of silica gel coated with 2,4-dinitrophenylhydrazine for collection of acrolein and HPLC analysis of the hydrazone derivatives.